What is the difference between a reagent blank and a sample blank?
• 2022/8/6
• Knowledge

 

Title
What is the difference between a reagent blank and a sample blank?

試劑空白和樣品空白有什麼不同？

 

Summary used
A sample blank is used to zero an instrument during a test procedure to correct for potential errors from existing color or turbidity in the sample before reagents are added. A reagent blank is a small positive error in test results caused by the reagents themselves.  樣品空白用於在測試過程中將儀器歸零，以校正在添加試劑之前樣品中現有顏色或濁度可能造成的誤差。試劑空白是指由試劑本身引起的測試數據中微小的正誤差。

 

Answer
A reagent blank refers to a small positive error in test results that comes from the reagents themselves. Hach Company makes every effort to manufacture reagents that have negligible blank values, and typically the values are so small that they do not affect test accuracy.  試劑空白是指由試劑本身引起的測試結果中微小的正誤差。我們竭盡全力製造空白值可忽略不計的試劑，通常這些空白值極小，不會影響測試準確度。

 

The reagent blank value is most important to measure and subtract from test results when measuring low concentrations. For example, subtracting a reagent blank value of 0.02 mg/L from a test result of 0.06 mg/L changes the result by more than 30 percent. On the other hand, subtracting a reagent blank value of 0.02 mg/L from a result of 1.23 mg/L changes the results by less than 2 percent.  在測量低濃度時，試劑空白值對於測量和從測試結果中扣除是最重要的。例如，從 0.06 mg/L 的測試結果中減去 0.02 mg/L 的試劑空白值，結果的偏差會超過 30%。另一方面，從 1.23 mg/L 的結果中減去 0.02 mg/L 的試劑空白值，結果的偏差不到 2%。

 

To measure the reagent blank, use good quality deionized water in place of your sample and run the test as usual, adding reagents and waiting any timed steps. Then subtract this value from your sample results. The reagent blank value can change from one reagent lot to the next. Therefore, measure the reagent blank each time you use a different lot of reagent.  要測量試劑空白，請使用優質的去離子水來代替樣品，並照常進行測試、添加試劑並等待所有計時步驟。然後從樣品結果中減去此值。試劑空白值可能會因試劑批次而異。因此，每次使用不同批次的試劑時，都應測量試劑空白。

 

A sample blank refers to using the sample for zeroing an instrument during a test procedure. A sample blank can correct for potential error from existing color or turbidity in the sample before reagents are added.  樣品空白是指在測試過程中使用樣品對儀器進行歸零。樣本空白可以校正樣品在添加試劑之前其既有顏色或濁度可能造成的誤差。

 

When zeroing the instrument on a sample blank, only the color that develops from reaction with the reagents is measured. Because background color and turbidity can vary from sample to sample, a sample blank is most commonly used to zero the instrument.  使用樣品空白對儀器進行歸零時，僅測量與試劑反應產生的顏色。由於不同樣品的背景顏色和濁度可能不同，因此最常使用樣品空白對儀器進行歸零。

 

 

Article Number
000006123

 

URL Name
KA-en-US-TE770-1000699